EPHA2 and WNT reciprocally regulate MAPK-dependent gene expression in a pancreatic cancer model

Shawn R. Wadia; Changyuan Hu; Siddhi Patnaik; Shreya Sridharan; Roger J. Daly; David M. Virshup; Babita Madan

doi:10.1172/jci.insight.202630

EPHA2 and WNT reciprocally regulate MAPK-dependent gene expression in a pancreatic cancer model

Shawn R. Wadia,¹ Changyuan Hu,² Siddhi Patnaik,¹ Shreya Sridharan,¹ Roger J. Daly,² David M. Virshup,¹ and Babita Madan¹

Published September 8, 2026 - More info

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Abstract

Wnt signaling drives tumorigenesis in multiple cancers, in part through complex interactions with other oncogenic pathways including the MAPK cascade. In Wnt-addicted cancers, pharmacologic and genetic inhibition of Wnt signaling activates multiple receptor tyrosine kinases (RTKs), increases ERK phosphorylation and induces MAPK target gene expression, but the specific RTKs responsible for this MAPK hyperactivation are not known. Here we performed phosphotyrosine-targeted mass spectrometry, which revealed robust phosphorylation of EPHA2 and EGFR upon Wnt inhibition. Unexpectedly, we find that in xenografts, EPHA2 suppresses EGFR and ERK activation. Most notably, the increased ERK phosphorylation observed in EPHA2 KO tumors is transcriptionally inert, as there is no concomitant increase in MAPK target gene expression until concomitant Wnt inhibition. This suggests a Wnt-activated transcriptional repressor such as GATA3 that gates MAPK signaling in Wnt-high cancers. While Wnt-high KRAS-mutant cancers are resistant to erlotinib alone, adding Wnt inhibitor mitigates this resistance. Additionally, loss of EPHA2 enhances their sensitivity to both erlotinib and Wnt inhibitors. These studies therefore identify therapeutic vulnerabilities in Wnt-high tumors, even within traditionally EGFR inhibitor-resistant, RAS-mutant contexts.

EPHA2 and WNT reciprocally regulate MAPK-dependent gene expression in a pancreatic cancer model

Shawn R. Wadia,¹ Changyuan Hu,² Siddhi Patnaik,¹ Shreya Sridharan,¹ Roger J. Daly,² David M. Virshup,¹ and Babita Madan¹

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EPHA2 and WNT reciprocally regulate MAPK-dependent gene expression in a pancreatic cancer model

Shawn R. Wadia,1 Changyuan Hu,2 Siddhi Patnaik,1 Shreya Sridharan,1 Roger J. Daly,2 David M. Virshup,1 and Babita Madan1

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Shawn R. Wadia,¹ Changyuan Hu,² Siddhi Patnaik,¹ Shreya Sridharan,¹ Roger J. Daly,² David M. Virshup,¹ and Babita Madan¹