Single-cell spatial profiling identifies a mucosal-like epithelial signature in Hidradenitis suppurativa tunnels
Peter Dimitrion, Jesse Veenstra, Deangelo Ferguson, Ping Wang, Jeffrey Cruz, Tasneem F. Mohammad, Ian Loveless, Aamir Siddiqui, Iltefat H. Hamzavi, Li Zhou, Indra Adrianto, Qing-Sheng Mi
Peter Dimitrion, Jesse Veenstra, Deangelo Ferguson, Ping Wang, Jeffrey Cruz, Tasneem F. Mohammad, Ian Loveless, Aamir Siddiqui, Iltefat H. Hamzavi, Li Zhou, Indra Adrianto, Qing-Sheng Mi
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Research Letter Dermatology Immunology

Single-cell spatial profiling identifies a mucosal-like epithelial signature in Hidradenitis suppurativa tunnels

Abstract

Authors

Peter Dimitrion, Jesse Veenstra, Deangelo Ferguson, Ping Wang, Jeffrey Cruz, Tasneem F. Mohammad, Ian Loveless, Aamir Siddiqui, Iltefat H. Hamzavi, Li Zhou, Indra Adrianto, Qing-Sheng Mi

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Figure 1

STEREOseq identifies HS tunnels bearing a mucosal epithelia phenotype.

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STEREOseq identifies HS tunnels bearing a mucosal epithelia phenotype. (...
(A) UMAP plot showing Louvain clustering (100 μm bins; left) and spatial projection (right). Red arrow indicates tunnel cluster. (B) Cluster marker genes enriched against GTEx_V8. (C) Unique and shared DEGs in tunnel vs. NL-epi and L-epi vs. NL-epi. (D and E) Top pathways from tunnel- and HS-L–specific genes, respectively. (F and G) Pseudocolored tissue sections by gene counts (20 μm bins): (F) whole tissue and (G) tunnel with overlying epidermis. (H) KRT13 expression in tunnels from HSOmicsDBv2. (I–L) KRT13 immunohistochemistry: (I) dermal tunnel, (J) lesional hair follicle infundibulum, (K) HC-epidermis (arrows indicate native melanin pigment), and (L) PL-epi. Total magnifcation: ×200 total magnification (I and J); ×40 total magnification (K and L). Statistical tests used were hypergeometric test for over-representation analysis (B, D, and E) and Student’s 2 sided t test (H).