786-0 cells were treated with decitabine or DMSO for 3 consecutive days; fresh media was added and harvested 24 hours later to obtain conditioned media. Conditioned media from 786-0 cells transfected with poly-IC was used as a positive control. Healthy donor T cells (n = 5) were cultured in conditioned media, and T cell function was assessed by flow cytometry. (A–D) T cells were stimulated with IL7, anti-CD3 antibody, and anti-CD3/CD28 antibodies and cultured for 5 days. Cell size, CD4, CD8, CD25, and CD71 was measured by flow cytometry to evaluate T cell activation. Surface expression of CD25 and CD71 (as median fluorescence intensity; MFI) was measured in CD8⁺ (A and B) and CD4⁺ (C and D) T cell subsets. (E and F) Migration of healthy donor T cells (n = 5) activated using anti-CD3/CD28 antibodies was assessed by Transwell assay. (E) Migration of activated T cells toward indicated conditioned medias. (F) Migration of activated T cells toward the indicated conditioned medias treated with either IGG isotype antibody, CXCL10 antibody, or CCL5 antibody (n = 5). Box-and-whisker plots show expression antiviral signaling genes in primary ccRCC cell lines (n = 5) treated with indicated doses of decitabine (or DMSO vehicle) as indicated by box color in key. Box ends represent the first and third quartiles, and the horizontal line inside the box corresponds to the median. The whiskers span from minimum or maximum values, and data points outside this range correspond to outliers. Significance assessed by paired t test, and P values were adjusted via Holm-Bonferroni correction. *P < 0.05.