Impaired autophagosome clearance contributes to local anesthetic bupivacaine-induced myotoxicity in mouse myoblasts
R Li, H Ma, X Zhang, C Li, J Xiong, T Lu, Y Mao… - …, 2015 - pubs.asahq.org
Background: The current study examined the role (s) of autophagy in myotoxicity induced by
bupivacaine in mouse myoblast C2c12 cells. Methods: C2c12 cells were treated with
bupivacaine. Myotoxicity was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-
diphenyltetrazolium bromide assay (n= 3 to 30), live/dead assay (n= 3 to 4), and
morphological alterations (n= 3). Autophagosome formation was reflected by microtubule-
associated protein light chain 3 conversion (n= 4 to 12) and light chain 3 punctation (n= 4 to …
bupivacaine in mouse myoblast C2c12 cells. Methods: C2c12 cells were treated with
bupivacaine. Myotoxicity was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-
diphenyltetrazolium bromide assay (n= 3 to 30), live/dead assay (n= 3 to 4), and
morphological alterations (n= 3). Autophagosome formation was reflected by microtubule-
associated protein light chain 3 conversion (n= 4 to 12) and light chain 3 punctation (n= 4 to …
