[HTML][HTML] Targeted Disruption of a Proximal Tubule–Specific TMEM174 Gene in Mice Causes Hyperphosphatemia and Vascular Calcification
S Miyazaki-Anzai, AL Keenan, J Blaine… - Journal of the …, 2022 - journals.lww.com
Background The proximal tubules play a critical role in phosphate (Pi) homeostasis by
reabsorbing Pi via sodium-dependent Pi cotransporters. NPT2A is a major proximal-specific
Pi cotransporter, whose expression is regulated by circulating hormones, such as
parathyroid hormone (PTH) and fibroblast growth factor 23 (FGF23). In this study, we aimed
to find a novel regulator in Pi homeostasis. Methods Using RNA-seq and RT-qPCR analysis,
we identified proximal tubule cell–enriched genes. We next used RNAi screening of the …
reabsorbing Pi via sodium-dependent Pi cotransporters. NPT2A is a major proximal-specific
Pi cotransporter, whose expression is regulated by circulating hormones, such as
parathyroid hormone (PTH) and fibroblast growth factor 23 (FGF23). In this study, we aimed
to find a novel regulator in Pi homeostasis. Methods Using RNA-seq and RT-qPCR analysis,
we identified proximal tubule cell–enriched genes. We next used RNAi screening of the …
