Smooth muscle protein 22α-Cre recombination in resting cardiac fibroblasts and hematopoietic precursors
S Ikeda, S Sugioka, T Kimura, N Ashida - Scientific Reports, 2022 - nature.com
The Cre-loxP system has been widely used for cell-or organ-specific gene manipulation, but
it is important to precisely understand what kind of cells the recombination takes place in.
Smooth muscle 22α (SM22α)-Cre mice have been utilized to alter genes in vascular smooth
muscle cells (VSMCs), activated fibroblasts or cardiomyocytes (CMs). Moreover, previous
reports indicated that SM22α-Cre is expressed in adipocytes, platelets or myeloid cells.
However, there have been no report of whether SM22α-Cre recombination takes place in …
it is important to precisely understand what kind of cells the recombination takes place in.
Smooth muscle 22α (SM22α)-Cre mice have been utilized to alter genes in vascular smooth
muscle cells (VSMCs), activated fibroblasts or cardiomyocytes (CMs). Moreover, previous
reports indicated that SM22α-Cre is expressed in adipocytes, platelets or myeloid cells.
However, there have been no report of whether SM22α-Cre recombination takes place in …
Author Correction: Smooth muscle protein 22α-Cre recombination in resting cardiac fibroblasts and hematopoietic precursors
S Ikeda, S Sugioka, T Kimura, N Ashida - Scientific Reports, 2022 - nature.com
SM22α-Cre recombination in vitro.(a) A representative bright field image of cultured
fibroblasts.(b) GFP expression in fibroblasts in vitro.(c) GFP expression in fibroblasts in vitro
(n= 3) and in vivo (n= 5).* p value< 0.01, evaluated by Student'st test.(d) GFP expression in
BMDMs with TGF-β stimulation.(e) No additional SM22α-Cre recombination in BMDMs with
TGF-β stimulation, which increases SM22α expression.(f) GFP expression in bone marrow-
derived cells with TGF-β stimulation.(g) No additional SM22α-Cre recombination in bone …
fibroblasts.(b) GFP expression in fibroblasts in vitro.(c) GFP expression in fibroblasts in vitro
(n= 3) and in vivo (n= 5).* p value< 0.01, evaluated by Student'st test.(d) GFP expression in
BMDMs with TGF-β stimulation.(e) No additional SM22α-Cre recombination in BMDMs with
TGF-β stimulation, which increases SM22α expression.(f) GFP expression in bone marrow-
derived cells with TGF-β stimulation.(g) No additional SM22α-Cre recombination in bone …
