[HTML][HTML] Determination of lymphoid cell fate is dependent on the expression status of the IL‐7 receptor
SJ Purohit, RP Stephan, HG Kim, BR Herrin… - The EMBO …, 2003 - embopress.org
SJ Purohit, RP Stephan, HG Kim, BR Herrin, L Gartland, CA Klug
The EMBO journal, 2003•embopress.orgSignaling through the IL‐7 receptor (IL‐7R) is necessary for the development of the earliest
B‐and T‐lineage cells. IL‐7R is first expressed on common lymphoid progenitor cells and is
not detected on primitive common myeloid progenitors. In this study, we show that enforced
expression of IL‐7R on multipotential stem cells does not influence lymphoid versus myeloid
cell fate. T cell development was compatible with sustained IL‐7R expression; however, we
observed a near complete block in B cell development at the onset of B‐lineage …
B‐and T‐lineage cells. IL‐7R is first expressed on common lymphoid progenitor cells and is
not detected on primitive common myeloid progenitors. In this study, we show that enforced
expression of IL‐7R on multipotential stem cells does not influence lymphoid versus myeloid
cell fate. T cell development was compatible with sustained IL‐7R expression; however, we
observed a near complete block in B cell development at the onset of B‐lineage …
Abstract
Signaling through the IL‐7 receptor (IL‐7R) is necessary for the development of the earliest B‐and T‐lineage cells. IL‐7R is first expressed on common lymphoid progenitor cells and is not detected on primitive common myeloid progenitors. In this study, we show that enforced expression of IL‐7R on multipotential stem cells does not influence lymphoid versus myeloid cell fate. T cell development was compatible with sustained IL‐7R expression; however, we observed a near complete block in B cell development at the onset of B‐lineage commitment. Unlike pre‐proB cells from control animals, developmentally‐arrested IL‐7R+ B220+ cd19− NK1. 1− Ly‐6C− cells failed to express EBF and Pax5. These results suggest that transient downregulation of IL‐7R signaling is a necessary event for induction of EBF and Pax5 expression and B‐lymphocyte commitment.
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