A photocleavable surfactant for top-down proteomics
Nature methods, 2019•nature.com
We report the identification of a photocleavable anionic surfactant, 4-
hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for
top-down proteomics. Azo can effectively solubilize proteins with performance comparable
to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-
aided top-down proteomics enables the solubilization of membrane proteins for
comprehensive characterization of post-translational modifications. Moreover, Azo is simple …
hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for
top-down proteomics. Azo can effectively solubilize proteins with performance comparable
to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-
aided top-down proteomics enables the solubilization of membrane proteins for
comprehensive characterization of post-translational modifications. Moreover, Azo is simple …
Abstract
We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS–polyacrylamide gel electrophoresis.
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