Therapeutic suppression of translation initiation modulates chemosensitivity in a mouse lymphoma model
J. Clin. Invest. Marie-Eve Bordeleau, et al. 118:2651 doi:10.1172/JCI34753 [Go to this article.]

Figure 1
CBFs inhibit cap-dependent translation. (A) Chemical structure of FA and silvestrol. (B) FA and silvestrol inhibit cap-dependent translation. Top: Schematic representation of the FF/HCV/Ren mRNA. Bottom: Dose-dependent inhibition of cap-dependent protein synthesis by FA and silvestrol in Krebs-2 translation extracts programmed with FF/HCV/Ren mRNA (10 μg/ml). Luciferase activity (RLU) results are expressed relative to values obtained in the presence of vehicle (MeOH) control. Results are expressed as mean ± SEM of 2 experiments. (C) A representative autoradiograph from in vitro translations performed in Krebs-2 extracts with [³⁵S]methionine and programmed with FF/HCV/Ren mRNA. The position of migration of FF and Ren proteins is indicated on the right.