Helicobacter pylori eradication shifts monocyte Fcγ receptor balance toward inhibitory FcγRIIB in immune thrombocytopenic purpura patients
J. Clin. Invest. Atsuko Asahi, et al. 118:2939 doi:10.1172/JCI34496 [Go to this article.]

Figure 4
Change in FcγRIIB expression levels in circulating monocytes from ITP patients before and after initiation of the H. pylori eradication. (A) FcγRIIA/IIB mRNA expression ratio on monocytes prior to treatment was determined by quantitative TaqMan PCR in 11 ITP patients infected with H. pylori and 5 uninfected ITP patients. The difference between the 2 groups was analyzed using the Mann-Whitney U test. (B) The FcγRIIA/IIB expression ratio on monocytes was serially measured prior to treatment and at 1 and 12 wk after initiation of the H. pylori eradication regimen in 6 H. pylori–positive ITP responders, 5 H. pylori–positive ITP nonresponders, and 5 H. pylori–negative ITP nonresponders. Results are shown as the mean + SD. Changes in the values at 1 and 12 wk from the baseline value taken at wk 0 were assessed by paired t test. **P < 0.01 compared with pre-treatment. Representative RT-PCR results for the expression of FcγRIIA, FcγRIIB, and GAPDH are shown for each patient group. (C) The protein expression of FcγRIIB on monocytes prior to treatment and 12 wk after initiation of the H. pylori eradication regimen in a representative H. pylori–positive responder and nonresponder. Open histograms show the cells stained with isotype-matched control Ab, and closed histograms show anti-FcγRIIB mAb–treated cells. (D) Expression levels of FcγRIIB on monocytes were measured prior to treatment and 12 wk after initiation of the H. pylori eradication regimen in 5 H. pylori–positive ITP responders, 4 H. pylori–positive ITP nonresponders, and 3 H. pylori–negative ITP nonresponders.