FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets
J. Clin. Invest. Naonobu Nishino, et al. 118:2808 doi:10.1172/JCI34090 [Go to this article.]

Figure 3
Characterization of adipose tissue in FSP27 knockout mice. (A) Comparison of interscapular brown fat pads (BAT) and epididymal white fat pads (WAT) of 14-week-old wild-type and FSP27-KO mice (left panel). Weights of epididymal WAT, subcutaneous WAT, and BAT isolated from 14-week-old wild-type and FSP27-KO mice (right panels). Data are mean ± SEM (n = 4–8). ^†P < 0.05 versus the corresponding value for wild type. (B) Sections of subcutaneous WAT and interscapular BAT from 14-week-old wild-type and FSP27-KO mice were stained with hematoxylin-eosin and examined by light microscopy. Scale bar: 20 μm. (C) Transmission electron microscopy of subcutaneous WAT and interscapular BAT from 6-week-old wild-type and FSP27-KO mice. L, lipid droplet; *, mitochondria. Scale bar: 500 nm. (D) Mitochondrial number (left panel) and size (right panel) in subcutaneous WAT, epididymal WAT, and interscapular BAT determined from electron micrographs similar to those in C. Mitochondrial number is expressed per nucleus and was determined for 25 wild-type and 28 FSP27-KO cells for subcutaneous WAT, 33 cells for epididymal WAT, and 56 wild-type and 64 FSP27-KO cells for BAT. Mitochondrial diameter was measured with a scale of 25 nm in 120 cells. Data are mean ± SEM. ^††P < 0.01 versus the corresponding value for wild type. (E) Southern blot analysis of total cellular DNA from BAT or WAT of 9-week-old wild-type or FSP27-KO mice with probes specific for the mitochondrial COXI gene and the nuclear 36B4 gene.