FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets
J. Clin. Invest. Naonobu Nishino, et al. 118:2808 doi:10.1172/JCI34090 [Go to this article.]

Figure 2
Glucose and energy metabolism in FSP27-knockout mice. (A) Body weight of wild-type, FSP27 heterozygous knockout (+/–), and FSP27-KO (–/–) mice maintained on a standard diet (SD) or fed a high-fat diet (HFD). Data are mean ± SEM (n = 7–11). ^§P < 0.05 versus corresponding values for wild-type or FSP27 heterozygous knockout mice on the high-fat diet; ^#P < 0.05 versus corresponding value for FSP27 heterozygous knockout mice on the high-fat diet. (B) Plasma glucose concentrations during an intraperitoneal glucose tolerance test (left panel) or an insulin tolerance test (right panel) in 12-week-old wild-type and FSP27-KO (KO) mice, either maintained on a standard diet or fed a high-fat diet from 4 weeks of age. Data are mean ± SEM (n = 11 for standard diet; n = 8 for high-fat diet.). *P < 0.05, **P < 0.01 versus corresponding value for wild-type animals on the standard diet; ^†P < 0.05, ^††P < 0.01 versus corresponding value for wild-type animals on the high-fat diet. (C) Hyperinsulinemic-euglycemic clamp analysis in 10- to 12-week-old wild-type and FSP27-KO mice fed a high-fat diet. GIR, glucose infusion rate; Rd, rate of glucose disappearance; BHGP, basal hepatic glucose production; CHGP, hepatic glucose production during the clamp period. Data are mean ± SEM (n = 6). ^§§P < 0.05, ^##P < 0.01 versus corresponding value for wild type. (D and E) Whole-body oxygen consumption rate (VO₂, expressed in milliliters per minute per gram of body weight) during a 12-hour dark/12-hour light cycle in 12-week-old mice fed a standard diet (n = 3 for wild type; n = 5 for FSP27-KO) (D) or a high-fat diet (n = 6) (E) is shown in the left panels. The average values for the 24-hour period are shown in the right panels. Data are mean ± SEM. ^‡P < 0.05, ^‡‡P < 0.01 versus wild type. (F and G) Average values of respiratory quotient (VCO₂/VO₂) (F) and energy expenditure (G) for the 24-hour period in the experiments shown in D and E. Data are mean ± SEM. ^‡P < 0.05, ^‡‡P < 0.01 versus wild type.