FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets
J. Clin. Invest. Naonobu Nishino, et al. 118:2808 doi:10.1172/JCI34090 [
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Figure 1Adipocyte-specific expression of
Fsp27.
(
A) Northern blot analysis of
FSP27 mRNA in 3T3-L1 cells at the indicated times after the onset of induction of adipocyte differentiation. The region of the ethidium bromide–stained gel containing 28S rRNA is also shown. (
B) Northern blot analysis of
FSP27 mRNA in 3T3-L1 adipocytes, either after incubation for 48 hours with 5 μM BRL49653 or TNF-α (10 ng/ml) or 48 hours after infection with adenoviral vectors encoding wild-type mouse PPARγ (adex-PPARγ-WT) or PPARγ-ΔC (adex-PPARγ-ΔC) or with the corresponding empty vector (adex-empty), at an MOI of 60 PFU per cell. (
C) Northern blot analysis of
FSP27 mRNA in various organs and tissues of C57BL/6J mice at 4 weeks of age. Sub, subcutaneous; Epi, epididymal. (
D) Immunoblot analysis of FSP27 in total lysates of WAT (subcutaneous or epididymal) and BAT isolated from C57BL/6J mice at 4 weeks of age. Both α-tubulin (loading control) and UCP-1 (BAT marker) were also examined. (
E) Northern blot analysis of
FSP27 mRNA in HB2 and HW cells at the indicated times after the onset of induction of adipocyte differentiation. (
F) Immunoblot analysis of FSP27 in HB2 and HW cells at the indicated times after the onset of induction of adipocyte differentiation using the antibodies to a COOH-terminal peptide of FSP27. Both α-tubulin and β-actin (loading controls) as well as COXIV (mitochondrial marker) were also examined.
