Adrenomedullin signaling is necessary for murine lymphatic vascular development
J. Clin. Invest. Kimberly L. Fritz-Six, et al. 118:40 doi:10.1172/JCI33302 [Go to this article.]

Figure 6
LECs of RAMP2^–/– mice are thin with otherwise normal ultrastructural features. Transmission electron microscopy of wild-type (A–C) and RAMP2^–/– (D–F) lymph sacs at E14.5. In general, the LECs of RAMP2^–/– mice appeared significantly thinner than those of wild-type littermates. Although obvious edema was present in the interstitial space surrounding both the veins and lymph sacs in RAMP2^–/– embryos, endothelial cells could be followed continuously around both vessel types, without obvious breaks or gaps. Other typical ultrastructural features of lymphatic vessels were identified in both wild-type and RAMP2^–/– mice including: overlapping contacts (arrowheads), tight junctions (arrows), and anchoring filaments (A). Occasionally necrotic endothelial cells (**) were observed in RAMP2^–/– mice, but the associated tight junctions remained structurally intact. n = 2 wild-type and 3 RAMP2^–/–. L; lumen. Scale bar: 1 μm.