Adrenomedullin signaling is necessary for murine lymphatic vascular development
J. Clin. Invest. Kimberly L. Fritz-Six, et al. 118:40 doi:10.1172/JCI33302 [
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Figure 5Loss of AM signaling results in hypoplastic lymph sacs. Lymph sacs of
calcrl–/–,
AM–/–, and
RAMP2–/– embryos were significantly smaller than those of their WT littermates at E13.5. (
A–
D) H&E-stained sections through transverse sections of the jugular lymph sacs of wild-type (
A),
calcrl–/– (
B)
, AM–/– (
C)
, and
RAMP2–/– (
D) embryos at E13.5. Note the remarkable decrease in size of lymph sacs relative to jugular vein and carotid artery in the AM signaling–mutant embryos, despite massive interstitial edema. (
E–
G) Quantitation of lymph sac area, normalized to jugular vein area in
calcrl–/– (
E)
, AM–/– (
F)
, and
RAMP2–/– (
G) embryos (black bars) and their wild-type littermates (gray bars) at E13.5.
E,
F, and
G have different scales.
n > 6 embryos per genotype. Error bars indicate SEM.
P values were determined using Student’s
s test. Original magnification, ×200.
