Adrenomedullin signaling is necessary for murine lymphatic vascular development
J. Clin. Invest. Kimberly L. Fritz-Six, et al. 118:40 doi:10.1172/JCI33302 [
Go to this article.]
Figure 4Differentiated lymph sacs and dermal lymphatics are present in AM signaling–null embryos.
Immunofluorescent staining of transverse sections through the jugular region (
A–
C) and sagittal sections through the skin (
D and
E) of E13.5 embryos. (
A) Endothelial cells of lymph sacs from wild-type mice at E13.5 expressed VEGFR3 (red) and Prox1 (green). (
B) Similar staining was observed in the lymph sacs of
RAMP2–/– littermates. Nuclei stained with Hoechst are indicated in blue (
A and
B). (
C) Prox1 (green) was also correctly coexpressed with PECAM (red) in lymph sacs of
AM–/– embryos. (
D and
E) Dermal lymphatics (arrows) were present in wild-type (
D) and
RAMP2–/– (
E) littermates at E14.5 as shown by VEGFR3 (red) and PECAM (green) staining. Dermal blood endothelium (arrowheads) expresses PECAM but not VEGFR3.
n > 6 embryos per genotype. A, carotid artery, A; S, skin. Original magnification, ×200. Scale bar: 100 μM.
