Adrenomedullin signaling is necessary for murine lymphatic vascular development
J. Clin. Invest. Kimberly L. Fritz-Six, et al. 118:40 doi:10.1172/JCI33302 [
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Figure 3AM signaling components are expressed in adult and developing lymphatic vessels. (
A) Isolated adult lymphatic vessels express
AM,
calcrl, and
RAMP2 at levels similar to whole embryo RNA extracts. Adult mouse lymphatic vessels were identified by the uptake of Evans blue dye injected into the hind paw and were subsequently microdissected for RNA isolation. Expression of
AM,
calcrl, and
RAMP2 mRNAs in these vessels was comparable to mRNA levels in E10–E12.5 embryos by quantitative RT-PCR.
n > 4 samples, analyzed twice in triplicate. (
B) To determine whether LECs express AM during development, we used an EGFP that serves as a biological marker of
AM gene expression in
AM gene–targeted mice (
35). Unstained transverse sections through the jugular region of an E12.5
AM+/– embryo show that AM is expressed in endothelial cells of both the jugular vein (V) and jugular lymph sac (LS) (arrows). Note the polarized expression of AM in the progenitor cells, which will eventually migrate to form the lymph sac. Original magnification, ×200.
