Adrenomedullin signaling is necessary for murine lymphatic vascular development
J. Clin. Invest. Kimberly L. Fritz-Six, et al. 118:40 doi:10.1172/JCI33302 [
Go to this article.]
Figure 1Phenotypic series of AM signaling–null mice. Genetic deletion of genes responsible for AM signaling results in a phenotypic series of null mice with generalized edema and lethality at mid-gestation. (
A) Generalized interstitial edema, without hemorrhage, was observed throughout
calcrl–/– embryos at E12.5. Similar edema was present by E13.5 in
AM–/– embryos (
D) and by E14.5 in RAMP2
–/– embryos (
G). Edema was not observed 1 day before onset, and no viable embryos were recovered 1–2 days after edema onset. H&E-stained transverse sections of wild-type littermates (
B,
E, and
H) and
calcrl–/–,
AM–/–, and
RAMP2–/– (
C,
F, and
I) embryos at E12.5, E13.5, and E14.5, respectively. Arrowheads indicate interstitial fluid accumulation. Original magnification, ×10 (
A,
D, and
G); ×20 (
B,
C,
E,
F,
H, and
I).
