Dopamine regulates endothelial progenitor cell mobilization from mouse bone marrow in tumor vascularization
J. Clin. Invest. Debanjan Chakroborty, et al. 118:1380 doi:10.1172/JCI33125 [Go to this article.]

Figure 6
Four-color flow cytometry showing presence of DA D₂ receptors on EPCs; DA inhibits VEGFA-induced migration of EPCs in vitro. (A–C) Respective isotype controls. (D) After excluding dead cells and debris, total CD45^– cells were gated (gate R2). (E) CD45^–cells were further analyzed for the presence of CD34 and VEGFR2. Upper-right quadrant represents CD45^–CD34⁺VEGFR2⁺ EPCs. These cells were further gated (gate R3) to analyze the presence of D₂ receptors. (F) Histogram showing the presence of D₂ receptors in 98% of the CD45^–CD34⁺VEGFR2⁺ EPCs. (G) Migration assay of cultured EPCs. DA was found to exert significant inhibition on VEGFA-induced migration of EPCs in a modified Boyden chamber. Treatment with eticlopride, a DA D₂ receptor antagonist, prior to DA treatment, completely abrogated the inhibitory effect of DA. Figures are representative of 4 separate experiments for each group. *P < 0.05.