Dopamine regulates endothelial progenitor cell mobilization from mouse bone marrow in tumor vascularization
J. Clin. Invest. Debanjan Chakroborty, et al. 118:1380 doi:10.1172/JCI33125 [Go to this article.]

Figure 3
DA inhibits incorporation of BM-derived cells into tumor vasculature and has no effect on the viability of CEPCs. (A and B) Tie2/lacZ transgenic BM cells were transplanted into lethally irradiated syngenic mice, followed by tumor transplantation. Tumor sections were incubated with X-gal and anti-CD31 to enumerate incorporation of lacZ⁺ BM-derived cells into tumor vasculature (arrows). (C) Percentage of β-gal–positive blood vessels. Figures are representative of 3 separate experiments. Scale bars: 50 μm. (D–K) DA had no effect on viability of CD45^–VEGFR2⁺ cells, including EPCs. Viability of cells was determined by flow cytometry based on 7AAD uptake. (D and E) Respective isotype controls. (F–K) After excluding dead cells, debris, and red blood cells, CD45^– cells were gated. (F, H, and J) VEGFR2⁺ plus 7AAD⁺ cells were quantitated out of the gated CD45^– cell population. (G, I, and K) Upper-right quadrants show the 7AAD⁺ nonviable cells among the CD45^–VEGFR2⁺ cells. Figures are representative of 4 separate experiments for each group.