CD200 is induced by ERK and is a potential therapeutic target in melanoma
J. Clin. Invest. Kimberly B. Petermann, et al. 117:3922 doi:10.1172/JCI32163 [Go to this article.]

Figure 3
shRNA-mediated knockdown of CD200 expression. (A) WM2664 human melanoma cells were transduced with a GFP-expressing human CD200 shRNA construct and stained with an anti-human CD200 antibody (red). Untransduced WM2664 cells expressed CD200 (red). Transduced WM2664 cells were negative for CD200 expression. Nuclei of cells are stained with DAPI (blue). Original magnification, ×40. (B) CD200 expression at different passages in culture after transduction was analyzed by flow cytometry. Efficient knockdown of the CD200 protein was observed after 6 passages (>15 days) in culture. (C) WM2664 human melanoma cells were FACS sorted for GFP expression and then analyzed by immunofluorescence for CD200 expression. WM2664 cells transduced with a CD200 shRNA were negative for CD200 expression. WM2664 cells transduced with a nonspecific shRNA showed intense staining for CD200 (red). Original magnification, ×10.